RNA
crRNA

Part:BBa_K2627003:Design

Designed by: Zhaoqin Zhang   Group: iGEM18_SDU-CHINA   (2018-10-06)


crRNA targeting GltA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

crRNA specifically targeting gltA gene and repress gene expression through type I-E CRISPR system

Source

crRNA originated in Escherichia coli EE-E15

References

Chang, Y., et al., Easy regulation of metabolic flux in Escherichia coli using an endogenous type I-E CRISPR-Cas system. Microbial Cell Factories, 2016. 15(1).